Evaluation of the MICRONAUT MIC-strip colistin assay for colistin susceptibility testing of carbapenem-resistant Acinetobacter baumannii and Enterobacterales

Hadas Kon; Maayan Amar Ben Dalak; David Schwartz; Yehuda Carmeli; Jonathan Lellouche

doi:10.1016/j.diagmicrobio.2021.115391

Evaluation of the MICRONAUT MIC-strip colistin assay for colistin susceptibility testing of carbapenem-resistant Acinetobacter baumannii and Enterobacterales

Diagn Microbiol Infect Dis. 2021 Aug;100(4):115391. doi: 10.1016/j.diagmicrobio.2021.115391. Epub 2021 Apr 16.

Authors

Hadas Kon¹, Maayan Amar Ben Dalak¹, David Schwartz¹, Yehuda Carmeli², Jonathan Lellouche³

Affiliations

¹ National Institute for Antibiotic Resistance and Infection Control, Ministry of Health, Israel.
² National Institute for Antibiotic Resistance and Infection Control, Ministry of Health, Israel; Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
³ National Institute for Antibiotic Resistance and Infection Control, Ministry of Health, Israel. Electronic address: jonathanl@tlvmc.gov.il.

PMID: 34077819
DOI: 10.1016/j.diagmicrobio.2021.115391

Abstract

We sought to evaluate the MICRONAUT MIC-Strip colistin (MMS) assay, a commercial broth microdilution (BMD) panel, using 273 carbapenem-resistant Acinetobacter baumannii and Enterobacterales isolates. BMD was used as gold standard. MMS had 98.5% sensitivity and 99.5% specificity. All 37 isolates with MICs close to the breakpoint exhibited complete agreement.

Keywords: Acinetobacter baumannii; Carbapenems resistance; Enterobacterales; MICRONAUT MIC-Strip colistinassay.

Publication types

Evaluation Study

MeSH terms

Acinetobacter baumannii / drug effects*
Anti-Bacterial Agents / pharmacology*
Colistin / pharmacology*
Drug Resistance, Multiple, Bacterial*
Enterobacter / drug effects*
Humans
Microbial Sensitivity Tests / instrumentation
Microbial Sensitivity Tests / standards*
Sensitivity and Specificity

Substances

Anti-Bacterial Agents
Colistin