A real time detection of the ovarian tumor associated antigen 1 (OVTA 1) in human serum by quartz crystal microbalance immobilized with anti-OVTA 1 polyclonal chicken IgY antibodies

S Sadhasivam; Jung-Chih Chen; S Savitha; Feng-Huei Lin; Yi-Yuan Yang; Chi-Hsin Lee

doi:10.1016/j.msec.2012.05.043

A real time detection of the ovarian tumor associated antigen 1 (OVTA 1) in human serum by quartz crystal microbalance immobilized with anti-OVTA 1 polyclonal chicken IgY antibodies

Mater Sci Eng C Mater Biol Appl. 2012 Oct 1;32(7):2073-2078. doi: 10.1016/j.msec.2012.05.043. Epub 2012 May 31.

Authors

S Sadhasivam¹, Jung-Chih Chen², S Savitha³, Feng-Huei Lin⁴, Yi-Yuan Yang⁵, Chi-Hsin Lee⁵

Affiliations

¹ Division of Medical Engineering Research, National Health Research Institutes, Taiwan, ROC; Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan, ROC; Department of Biomedical Imaging and Radiological Sciences, China Medical University, Taichung, Taiwan, ROC.
² Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan, ROC; Medical Device Innovation Center, National Cheng Kung University, Tainan,Taiwan, ROC.
³ Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan, ROC; School of Medical Laboratory Science and Biotechnology, Taipei Medical University, Taipei, Taiwan, ROC.
⁴ Division of Medical Engineering Research, National Health Research Institutes, Taiwan, ROC; Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan, ROC. Electronic address: double@ntu.edu.tw.
⁵ School of Medical Laboratory Science and Biotechnology, Taipei Medical University, Taipei, Taiwan, ROC.

PMID: 34062698
DOI: 10.1016/j.msec.2012.05.043

Abstract

In this study, we developed a sensitive quartz crystal microbalance (QCM) sensor which employs the anti-ovarian tumor associated antigen 1 (OVTA 1) IgY polyclonal chicken antibodies on the crystal surface for the detection of OVTA 1 in human serum. The anti-OVTA 1 IgY antibodies were anchored on the thiol-activated sensor surface using 1-ethyl-3[3-dimethylaminopropyl] carbodiimide hydrochloride/N-hydroxysuccinimide (EDC-NHS) coupling. The responses for different concentrations of anti-OVTA 1 antibody were also studied and 40μg/mL was favorable for homogenous antibody coverage. The QCM sensor was used to monitor both immobilization and the target binding affinity. The anti-OVTA 1 antibody based QCM has allowed the competent detection of OVTA 1 in a high linear range of 0.5-10μg/mL. The total time needed to complete the detection was as short as 5-6h. The regeneration studies demonstrated that the proposed sensor was reusable up to 9cycles with a slight loss in binding affinity. The detection of OVTA 1 in human serum allows a potential exploitation of the anti-OVTA 1 polyclonal antibody based QCM immunoassay for the screening of OVTA 1 antigen.

Keywords: Human serum; Immunosensors; OVTA 1 antigen; Ovarian cancer; Polyclonal chicken IgY antibodies; QCM.