Comparison of the amino acid sequences of human protamines HP2 and HP3 and of intermediate basic nuclear proteins HPS1 and HPS2. Structural evidence that HPS1 and HPS2 are pro-protamines

P Sautière; A Martinage; D Bélaiche; A Arkhis; P Chevaillier

Comparison of the amino acid sequences of human protamines HP2 and HP3 and of intermediate basic nuclear proteins HPS1 and HPS2. Structural evidence that HPS1 and HPS2 are pro-protamines

J Biol Chem. 1988 Aug 15;263(23):11059-62.

Authors

P Sautière¹, A Martinage, D Bélaiche, A Arkhis, P Chevaillier

Affiliation

¹ Unité Associée Centre National de la Recherche Scientifique No. 409, Institut de Recherches sur le Cancer, Lille, France.

PMID: 3403514

Abstract

Two intermediate nuclear basic proteins HPS1 and HPS2 were isolated from human sperm. They were characterized by their electrophoretic mobility in acid-urea gels, their amino acid composition, and their peptide maps after digestion by endoproteinase Lys-C and by endoproteinase Glu-C. Their amino-terminal amino acid sequences have also been determined. The structural data thus obtained suggest that HPS1 and HPS2 are precursors of human protamines HP2 and HP3.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Amino Acids / analysis
Electrophoresis, Polyacrylamide Gel
Humans
Molecular Sequence Data
Nuclear Proteins / analysis*
Protamines / analysis*

Substances

Amino Acids
Nuclear Proteins
Protamines
protamine 2
protamine P2
protamine P3