Characterization of Tempranillo negro (VN21), a high phenolic content grapevine Tempranillo clone, through UHPLC-QqQ-MS/MS polyphenol profiling

Food Chem. 2021 Oct 30:360:130049. doi: 10.1016/j.foodchem.2021.130049. Epub 2021 May 11.

Abstract

Grapevine cultivar and clone genotype is an important factor in the phenolic composition of wine. In this study, a new intense dark black berry color variant of Tempranillo, known as Tempranillo negro or VN21, is described. A targeted chromatographic approach based on UHPLC-QqQ-MS/MS was used to study the anthocyanins and non-colored phenols of the grape berry (skin and seeds) and wine. RJ43, one of the most cultivated clones in D.O.Ca. Rioja (Spain), was analyzed for comparison. Results suggest that the unique color of the grape skin in Tempranillo negro could be explained by higher concentrations of peonidin and cyanidin derivatives. This genotype accumulated anthocyanins in the seeds. Those differences in the berry were enhanced in the VN21 wines, which displayed notably higher concentrations of anthocyanins, and significantly increased contents of proanthocyanidins and stilbenes. This study exemplifies the application of phenol chromatographic analyses of spontaneous somatic variants to grapevine clonal selection.

Keywords: Anthocyanins; Berry phenolic composition; Phenolic compounds; Somatic variation; Stilbenes; Tempranillo; Vitis vinifera; Wine phenolic composition.

MeSH terms

  • Anthocyanins / analysis
  • Chromatography, High Pressure Liquid
  • Fruit / chemistry*
  • Polyphenols / analysis*
  • Proanthocyanidins / analysis
  • Seeds / chemistry
  • Stilbenes / analysis
  • Tandem Mass Spectrometry
  • Vitis / chemistry*
  • Wine / analysis*

Substances

  • Anthocyanins
  • Polyphenols
  • Proanthocyanidins
  • Stilbenes
  • cyanidin
  • peonidin