The present results give evidence by using cytochemical markers of motor end-plates (ChE, AchR) and ultrastructural techniques that the Levator ani (LA) muscle is also present in adult females: it is composed of differentiated and innervated fibres. A significant difference, both on the number of fibres per muscle (n) and on their average cross-sectional area (acsa), was observed between sexes:-male: n = 5300 +/- 687, acsa: 522 +/- 68.6 microns 2;-female: n = 565 +/- 246.9, acsa: 68 +/- 8.6 microns 2. These results suggest that testosterone could control, at least partially, the number and the diameter of muscle fibres during development.