Trips-Viz: an environment for the analysis of public and user-generated ribosome profiling data

Nucleic Acids Res. 2021 Jul 2;49(W1):W662-W670. doi: 10.1093/nar/gkab323.

Abstract

Trips-Viz (https://trips.ucc.ie/) is an interactive platform for the analysis and visualization of ribosome profiling (Ribo-Seq) and shotgun RNA sequencing (RNA-seq) data. This includes publicly available and user generated data, hence Trips-Viz can be classified as a database and as a server. As a database it provides access to many processed Ribo-Seq and RNA-seq data aligned to reference transcriptomes which has been expanded considerably since its inception. Here, we focus on the server functionality of Trips-viz which also has been greatly improved. Trips-viz now enables visualisation of proteomics data from a large number of processed mass spectrometry datasets. It can be used to support translation inferred from Ribo-Seq data. Users are now able to upload a custom reference transcriptome as well as data types other than Ribo-Seq/RNA-Seq. Incorporating custom data has been streamlined with RiboGalaxy (https://ribogalaxy.ucc.ie/) integration. The other new functionality is the rapid detection of translated open reading frames (ORFs) through a simple easy to use interface. The analysis of differential expression has been also improved via integration of DESeq2 and Anota2seq in addition to a number of other improvements of existing Trips-viz features.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Mass Spectrometry
  • Open Reading Frames
  • Protein Biosynthesis*
  • Proteomics
  • RNA-Seq
  • Ribosomes* / metabolism
  • Sequence Analysis, RNA / methods*
  • Software*