Abstract
5-Fluorouracil (5-FU) resistance is an urgent problem of colorectal cancer (CRC) chemotherapy that needs to be resolved. To investigate 5-FU-associated lncRNAs for CRC might be of great significant. LncRNA ENSG00000254615 was detected by RNA-sequencing. ENSG00000254615 were detected highly expressed in 5-FU-sensitive CRC cells and tissue specimens, and inhibited cell proliferation and attenuated 5-FU resistance in vitro and in vivo. Furthermore, ENSG00000254615 participated in the regulation of p21 and Cyclin D1. Taken together, we proposed that ENSG00000254615 inhibits proliferation and attenuates 5-FU resistance of CRC by regulating p21 and Cyclin D1 expression.
Keywords:
5-FU resistance; Colorectal cancer; lncRNA; proliferation.
MeSH terms
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Animals
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Antimetabolites, Antineoplastic / pharmacology
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Cell Line, Tumor
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Cell Proliferation / genetics*
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Colorectal Neoplasms / drug therapy
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Colorectal Neoplasms / genetics*
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Colorectal Neoplasms / metabolism
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Cyclin D1 / genetics*
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Cyclin D1 / metabolism
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Cyclin-Dependent Kinase Inhibitor p21 / genetics*
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Cyclin-Dependent Kinase Inhibitor p21 / metabolism
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Drug Resistance, Neoplasm / genetics*
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Fluorouracil / pharmacology*
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Gene Expression Regulation, Neoplastic*
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HCT116 Cells
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HEK293 Cells
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Humans
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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RNA, Long Noncoding / genetics*
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Tumor Burden / drug effects
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Tumor Burden / genetics
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Xenograft Model Antitumor Assays / methods
Substances
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Antimetabolites, Antineoplastic
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CDKN1A protein, human
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Cyclin-Dependent Kinase Inhibitor p21
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RNA, Long Noncoding
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Cyclin D1
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Fluorouracil