Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system

Xiuqing Xiao; Yashuang Chen; Lisheng Peng; Tian Zhang

doi:10.1016/j.scr.2021.102309

Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system

Stem Cell Res. 2021 May:53:102309. doi: 10.1016/j.scr.2021.102309. Epub 2021 Mar 24.

Authors

Xiuqing Xiao¹, Yashuang Chen¹, Lisheng Peng², Tian Zhang³

Affiliations

¹ Department of Neurology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
² Department of Neurology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China. Electronic address: lishengpeng79@163.com.
³ Medical Research Center, People's Hospital of Longhua, Shenzhen 518109, China. Electronic address: baicao27@163.com.

PMID: 33799274
DOI: 10.1016/j.scr.2021.102309

Abstract

Human ALX1 gene (ALX Homeobox 1) is a protein coding gene and gene ontology annotations related to this gene include DNA-binding transcription factor activity and protein heterdimerization activity. It is necessary for survival of forebrain mesenchyme and may be involved in development of cervix. However, the function of the gene has yet to be determined in humans. Here we generated an ALX1 homozygous human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system. The WAe001-A-060 has a normal undifferentiated morphology and karyotype, pluripotency and three germ layers differentiation potential in vivo.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems / genetics
Cell Line
Clustered Regularly Interspaced Short Palindromic Repeats
Embryonic Stem Cells
Female
Human Embryonic Stem Cells*
Humans