Characterization of Brucella abortus S19 as a challenge strain for use in a mouse model of brucellosis

Jessica M Jacob; Roy Curtiss 3rd

doi:10.1016/j.micinf.2021.104809

Characterization of Brucella abortus S19 as a challenge strain for use in a mouse model of brucellosis

Microbes Infect. 2021 May-Jun;23(4-5):104809. doi: 10.1016/j.micinf.2021.104809. Epub 2021 Mar 19.

Authors

Jessica M Jacob¹, Roy Curtiss 3rd²

Affiliations

¹ Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, 2015 SW 16th Ave, Gainesville, FL, 32608, USA. Electronic address: jessicamjacob@ufl.edu.
² Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, 2015 SW 16th Ave, Gainesville, FL, 32608, USA. Electronic address: rcurtiss@ufl.edu.

PMID: 33753207
DOI: 10.1016/j.micinf.2021.104809

Abstract

The objective of this project was to conduct a feasibility study to determine whether the Brucella abortus S19 vaccine infects and persists in mice and determine whether S19 can be used as a challenge strain for vaccine trial studies. Groups of BALB/c mice were inoculated (intraperitoneally, subcutaneously, intranasally) and euthanized to determine colonization titers in the spleens and lungs. This study showed that S19 does infect and persist in the tissues of mice for 8 weeks and demonstrates that S19 can be used, safely and economically under BSL2 containment, as the challenge strain for future trials to evaluate vaccine efficacy.

Keywords: Bacterial loads; Biosafety; Brucella abortus; Mice; Spleen; Vaccine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brucella abortus / classification*
Brucella abortus / physiology*
Brucellosis / microbiology*
Brucellosis / pathology
Disease Models, Animal*
Female
Mice
Mice, Inbred BALB C
Specific Pathogen-Free Organisms