Rapid and efficient generation of T-cell receptor-like antibodies using chip-based single-cell analysis

Tatsuhiko Ozawa; Eiji Kobayashi; Hiroshi Hamana; Tomoko Nakamura; Fulian Lyu; Atsushi Hayashi; Atsushi Muraguchi; Hiroyuki Kishi

doi:10.1002/eji.202049083

Rapid and efficient generation of T-cell receptor-like antibodies using chip-based single-cell analysis

Eur J Immunol. 2021 Jul;51(7):1850-1853. doi: 10.1002/eji.202049083. Epub 2021 Mar 29.

Authors

Tatsuhiko Ozawa¹, Eiji Kobayashi¹, Hiroshi Hamana¹, Tomoko Nakamura², Fulian Lyu^{3

4}, Atsushi Hayashi², Atsushi Muraguchi¹, Hiroyuki Kishi¹

Affiliations

¹ Department of Immunology, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan.
² Department of Ophthalmology, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan.
³ Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences (Medicine), University of Toyama, Toyama, Japan.
⁴ Department of Hematology, Collaborative Innovation Center of Hematology, The First Affiliated Hospital of Soochow University, Soochow University, Taipei, Taiwan, P. R. China.

PMID: 33728647
DOI: 10.1002/eji.202049083

Abstract

Generation of TCR-like monoclonal antibodies using conventional methods is markedly laborious and inefficient. We have proposed improvements of ISAAC (chip-based Ab-secreting cell [ASC] screening method), allows comprehensive analysis of ASCs at the single-cell level to obtain TCR-like antibodies; blocking procedure enables us to avoid the detection of non-TCR-like antibodies.

Keywords: ISAAC; TCR; TCR-like antibody; tumor immunotherapy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal / immunology*
Humans
Receptors, Antigen, T-Cell / immunology*
Single-Cell Analysis / methods

Substances

Antibodies, Monoclonal
Receptors, Antigen, T-Cell