Nono-titanium dioxide exposure during the adolescent period induces neurotoxicities in rats: Ameliorative potential of bergamot essential oil

Abstract

Introduction: In adolescence, the brain is still maturing, and disorders in maturation may affect the normal development of the brain. Exposure to titanium dioxide nanoparticles (TiO₂ NPs) has various potential negative effects on the central nervous system. Bergamot essential oil (BEO) has been found to be effective for neuroprotection.

Methods: The rats were injected intraperitoneally with TiO₂ NPs (20 mg/kg) and/or BEO (200 mg/kg). The endogenous antioxidant state and inflammatory parameters were estimated using ELISA kits, and then the memory ability and anxiety-like behavior in rats were assessed.

Results: TiO₂ NPs exposure during the adolescent period induced anxiety-like behavior, cognitive impairment, neuroinflammation and oxidative damage in hippocampus, and BEO treatment could significantly ameliorate the neurotoxicities induced by TiO₂ NPs exposure.

Conclusion: Our results suggest that the negative effects of TiO₂ NPs exposure during the adolescent period on anxiety-like behavior and cognitive function may be related to oxidative stress and neuroinflammation induced by TiO₂ NPs exposure. In addition, BEO may ameliorate the neurotoxicities induced by TiO2 NPs exposure in adolescent rats through the antioxidant and anti-inflammatory activity of BEO.

Keywords: Nano-titanium dioxide; anxiety; bergamot essential oil; memory; neurotoxicity.