Case Report: Identification of SARS-CoV-2 in Cerebrospinal Fluid by Ultrahigh-Depth Sequencing in a Patient With Coronavirus Disease 2019 and Neurological Dysfunction

Pan Xiang; Xinmin Xu; Xin Lu; Lili Gao; Huizhu Wang; Zhenpeng Li; Haofeng Xiong; Ruihong Li; Yanwen Xiong; Lin Pu; Tian Qin; Fangfang Jin; Hongyu Ren; Chuansheng Li; Jing Yang; Ming Zhang; Jie Gong; Xiaoping Chen; Han Zheng; Jianbo Tan; Yao Sun; Fei Zhao; Xuexin Hou; Yufeng Liu; Hebing Guo; Jingjing Hao; Biao Kan; Haijian Zhou; Yajie Wang; Jingyuan Liu

doi:10.3389/fmed.2021.629828

Case Report: Identification of SARS-CoV-2 in Cerebrospinal Fluid by Ultrahigh-Depth Sequencing in a Patient With Coronavirus Disease 2019 and Neurological Dysfunction

Front Med (Lausanne). 2021 Feb 22:8:629828. doi: 10.3389/fmed.2021.629828. eCollection 2021.

Authors

Pan Xiang¹, Xinmin Xu², Xin Lu³, Lili Gao¹, Huizhu Wang², Zhenpeng Li³, Haofeng Xiong¹, Ruihong Li², Yanwen Xiong³, Lin Pu¹, Tian Qin³, Fangfang Jin², Hongyu Ren³, Chuansheng Li¹, Jing Yang³, Ming Zhang¹, Jie Gong³, Xiaoping Chen³, Han Zheng³, Jianbo Tan¹, Yao Sun¹, Fei Zhao³, Xuexin Hou³, Yufeng Liu¹, Hebing Guo¹, Jingjing Hao¹, Biao Kan³, Haijian Zhou³, Yajie Wang², Jingyuan Liu¹

Affiliations

¹ Department of Critical Care Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing, China.
² Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing, China.
³ State Key Laboratory of Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Beijing, China.

Abstract

We reported that the complete genome sequence of SARS-Coronavirus-2 (SARS-CoV-2) was obtained from a cerebrospinal fluid (CSF) sample by ultrahigh-depth sequencing. Fourteen days after onset, seizures, maxillofacial convulsions, intractable hiccups and a significant increase in intracranial pressure developed in an adult coronavirus disease 2019 patient. The complete genome sequence of SARS-CoV-2 obtained from the cerebrospinal fluid indicates that SARS-CoV-2 can invade the central nervous system. In future, along with nervous system assessment, the pathogen genome detection and other indicators are needed for studying possible nervous system infection of SARS-CoV-2.

Keywords: COVID-19; SARS-CoV-2; central nervous system; infection; metagenomic next-generation sequencing.

Publication types

Case Reports