Silk fibroin/polyurethane patch implantation in hyperglycemic rat model

J Biomater Appl. 2021 Oct;36(4):701-713. doi: 10.1177/0885328221999227. Epub 2021 Mar 2.

Abstract

Purpose: To understand the complication and histopathological characteristics between the Silk Fibroin/Polyurethanes (SF/PU) and the host response, and to unveil the compatibility of the patch in diabetes individuals.

Methods: Rats were divided into DM and control (CT) groups, and the DM group was induced with streptozotocin. All groups underwent the SF/PU patch implantation in the abdominal aorta, and the implanted patches were evaluated at one, two, three, and four weeks after implantation.

Results: DM group had more fibrosis formation and a delayed endothelialization compared to the CT group. There was no evidence of chronic inflammation in both DM and CT groups.

Conclusions: Fibrosis in hyperglycemic individuals could promote the formation of new vascular structures in the implanted patch such as endothelial and vascular smooth muscle cells. In summary, the SF/PU patch was no serious complications when implanted under hyperglycemia, and the patch was suitable to implant in diabetes mellitus.

Keywords: Diabetes mellitus; endothelialization; patch; polyurethane; silk fibroin.

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry*
  • Blood Vessels
  • Fibroins / chemistry*
  • Hyperglycemia
  • Male
  • Materials Testing
  • Polyurethanes / chemistry*
  • Rats
  • Rats, Sprague-Dawley
  • Silk / chemistry*
  • Tissue Engineering

Substances

  • Biocompatible Materials
  • Polyurethanes
  • Silk
  • Fibroins