An efficient heterologous Escherichia coli-based expression system for lectin production from Pleurocybella porrigens

Abstract

In this study, we report a more efficient heterologous expression of lectin from Pleurocybella porrigens (PPL) using an Escherichia coli-based expression system. The yield (9.3 mg/L culture broth) of recombinant PPL (rPPL) using this expression system was increased approximately 9-fold compared to our previous study. The rPPL obtained in this study exhibited the same biochemical properties as the native PPL.

Keywords: Escherichia coli; Pleurocybella porrigens; Heterologous expression; lectin.