Long-Chain Noncoding RNA ADAMTS9-AS2 Regulates Proliferation, Migration, and Apoptosis in Bladder Cancer Cells Through Regulating miR-182-5p

J Interferon Cytokine Res. 2021 Feb;41(2):60-71. doi: 10.1089/jir.2020.0137.

Abstract

The long-chain noncoding RNA ADAMTS9-AS2 functions as a tumor suppressor gene in many cancers. However, the underlying mechanism remains to be fully elucidated in bladder cancer (BC). ADAMTS9-AS2 exhibited a lower expression level in BC samples and cell lines. In addition, overexpression of ADAMTS9-AS2 obviously suppressed proliferation and migration, and induced apoptosis of T24 cells, while transfection with the ADAMTS9-AS2 inhibitor had opposite results in 5637 cells. Furthermore, miR-182-5p was the target microRNA of ADAMTS9-AS2 and was negatively correlated with ADAMTS9-AS2 expression. Upregulation of miR-182-5p reversed the effects of ADAMTS9-AS2 overexpression on biological function in T24 cells. ADAMTS9-AS2 was a tumor suppressor that inhibited BC cell proliferation and induced cellular apoptosis by targeting miR-182-5p, and it could be a promising target for BC treatment.

Keywords: ADAMTS9-AS2; apoptosis; bladder cancer; miR-182-5p; proliferation.

MeSH terms

  • Apoptosis
  • Cell Movement
  • Cell Proliferation
  • Cell Survival
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • RNA, Long Noncoding / genetics
  • RNA, Long Noncoding / metabolism*
  • Tumor Cells, Cultured
  • Urinary Bladder Neoplasms / metabolism*
  • Urinary Bladder Neoplasms / pathology

Substances

  • MicroRNAs
  • Mirn182 microRNA, human
  • RNA, Long Noncoding