Cross-linked arabinoxylan in a Ca2+-alginate matrix reversed the body weight gain of HFD-fed C57BL/6J mice through modulation of the gut microbiome

Abstract

Here, we compared the effects of different physical forms of arabinoxylan (AX) - a microsphere of cross-linked arabinoxylan (CAX) in a Ca²⁺-alginate matrix (MC) and physical mixture of AX and alginate (PM) on gut microbiota and development of obesity in C57BL/6J mice. Supplementation of MC in high fat (HF) diet to mice for 10 weeks significantly reversed the body weight gain induced by the HF diet, along with less fat accumulation in both livers and the epididymal adipose than the PM group. Microbiome analysis showed that MC significantly altered the gut microbiota composition with a noticeable increase of butyrogenic bacteria of Lachnospiraceae. The butyrate produced by MC fermentation and the increased abundance of Lachnospiraceae might be the underlying mechanism of the anti-obesity effect of MC. The results indicated that the physical forms of dietary fiber are closely associated with its health benefits, and MC might be served as a new functional food ingredient to prevent obesity.

Keywords: Anti-obesity; Ca(2+)-alginate matrix; Cross-linking arabinoxylan; Gut microbiota; Physical form.