Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice

Elife. 2021 Feb 2:10:e62174. doi: 10.7554/eLife.62174.

Abstract

Heart failure is a major public health problem, and abnormal iron metabolism is common in patients with heart failure. Although iron is necessary for metabolic homeostasis, it induces a programmed necrosis. Iron release from ferritin storage is through nuclear receptor coactivator 4 (NCOA4)-mediated autophagic degradation, known as ferritinophagy. However, the role of ferritinophagy in the stressed heart remains unclear. Deletion of Ncoa4 in mouse hearts reduced left ventricular chamber size and improved cardiac function along with the attenuation of the upregulation of ferritinophagy-mediated ferritin degradation 4 weeks after pressure overload. Free ferrous iron overload and increased lipid peroxidation were suppressed in NCOA4-deficient hearts. A potent inhibitor of lipid peroxidation, ferrostatin-1, significantly mitigated the development of pressure overload-induced dilated cardiomyopathy in wild-type mice. Thus, the activation of ferritinophagy results in the development of heart failure, whereas inhibition of this process protects the heart against hemodynamic stress.

Keywords: autophagy; biochemistry; chemical biology; ferritin; heart failure; iron; medicine; mouse; necrosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aorta
  • Autophagy
  • Cardiomyopathies / drug therapy
  • Constriction
  • Cyclohexylamines / pharmacology
  • Disease Models, Animal
  • Ferritins / genetics
  • Ferritins / metabolism
  • Heart Failure / drug therapy
  • Heart Failure / etiology*
  • Iron / metabolism
  • Lipid Peroxidation
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Myocytes, Cardiac / drug effects
  • Myocytes, Cardiac / metabolism
  • Nuclear Receptor Coactivators / genetics*
  • Nuclear Receptor Coactivators / metabolism*
  • Phenylenediamines / pharmacology

Substances

  • Cyclohexylamines
  • NcoA4 protein, mouse
  • Nuclear Receptor Coactivators
  • Phenylenediamines
  • ferrostatin-1
  • Ferritins
  • Iron