CYLD mediates human pulmonary artery smooth muscle cell dysfunction in congenital heart disease-associated pulmonary arterial hypertension

J Cell Physiol. 2021 Sep;236(9):6297-6311. doi: 10.1002/jcp.30298. Epub 2021 Jan 28.

Abstract

Pulmonary arterial hypertension (PAH) is a common complication of congenital heart disease (CHD). Deubiquitinase cylindromatosis (CYLD) has been reported to significantly aggravate vascular smooth muscle cell (VSMC) phenotypic transformation, proliferation, and migration. Here, we aimed to further investigate its roles and underlying mechanisms in the CHD-PAH development. The expression of CYLD in the lung tissues from CHD-PAH patients and monocrotaline (MCT) plus aortocaval (AV)-induced PAH rats, pulmonary artery smooth muscle cells (PASMCs) from MCT-AV-induced PAH rats, and human PASMCs (HPASMCs) was evaluated. After infection with CYLD siRNA or pcNDA3.1-CYLD, the proliferation, migration, and apoptosis of HPASMCs were measured using an EdU assay, transwell and scratch wound healing assays, and flow cytometric assay, respectively. An adeno-associated virus (AAV) vector encoding CYLD was used to suppress CYLD expression by being intratracheally instilled in rats 7 days before MCT-AV treatment. The results showed that CYLD was increased in the lung tissues from CHD-PAH patients and MCT-AV-induced PAH rats, and in PASMCs from MCT-AV-induced PAH rats. The contractile-type HPASMCs expressed low levels of CYLD, while the proliferative synthetic-type HPASMCs expressed high levels of CYLD. In addition, CYLD could mediate HPASMC dysfunction, which regulated HPASMC phenotypic transformation and proliferation via the modulation of p38 and ERK activation, while CYLD regulated HPASMC migration via the modulation of p38 activation. In vivo results demonstrated that the local suppression of CYLD expression could attenuate the increased levels of PAH and its associated pulmonary vascular remodeling in MCT-AV-induced PAH rats. Collectively, these results indicated that CYLD might be a potential novel therapeutic target for the prevention of PAH and pulmonary vascular remodeling in CHD-PAH through the modulation of HPASMC dysfunction.

Keywords: congenital heart disease; cylindromatosis; human pulmonary artery smooth muscle cells; pulmonary arterial hypertension.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Animals
  • Apoptosis
  • Biomarkers / metabolism
  • Cell Movement
  • Cell Proliferation
  • Child
  • Child, Preschool
  • Deubiquitinating Enzyme CYLD / metabolism*
  • Female
  • Heart Defects, Congenital / complications*
  • Heart Defects, Congenital / physiopathology
  • Hemodynamics
  • Humans
  • Lung / pathology
  • MAP Kinase Signaling System
  • Male
  • Middle Aged
  • Monocrotaline
  • Myocytes, Smooth Muscle / pathology*
  • NF-kappa B / metabolism
  • Phenotype
  • Pulmonary Arterial Hypertension / complications*
  • Pulmonary Arterial Hypertension / physiopathology*
  • Pulmonary Artery / pathology*
  • Rats
  • Rats, Sprague-Dawley
  • Serum
  • Ubiquitin Thiolesterase / metabolism
  • Vascular Remodeling
  • Young Adult

Substances

  • Biomarkers
  • NF-kappa B
  • Monocrotaline
  • CYLD protein, human
  • CYLD protein, rat
  • Deubiquitinating Enzyme CYLD
  • Ubiquitin Thiolesterase