Abstract
Protein kinase C activity in lymphocytes was down-regulated upon exposure of the cells to 12-0-tetradecanoylphorbol-13 acetate. This down-regulation was prevented by preincubating the cells with sphingosine, a reported protein kinase C inhibitor. Two other protein kinase C inhibitors, palmitoylcarnitine and phloretin, were ineffective in preventing down-regulation by the phorbol ester.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cattle
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Lymphocytes / drug effects
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Lymphocytes / enzymology*
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Palmitoylcarnitine / pharmacology
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Phloretin / pharmacology
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Protein Kinase C / antagonists & inhibitors
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Protein Kinase C / metabolism*
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Sphingosine / pharmacology*
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Tetradecanoylphorbol Acetate / pharmacology*
Substances
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Palmitoylcarnitine
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Protein Kinase C
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Sphingosine
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Tetradecanoylphorbol Acetate
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Phloretin