1α,25-dihydroxyvitamin D3 promotes early osteogenic differentiation of PDLSCs and a 12-year follow-up case of early-onset vitamin D deficiency periodontitis

J Steroid Biochem Mol Biol. 2021 Apr:208:105805. doi: 10.1016/j.jsbmb.2020.105805. Epub 2021 Jan 21.

Abstract

Periodontitis is a chronic periodontal disease that contributes to tooth loss. In recent years, many animal studies have reported that vitamin D (VitD) deficiency results in chronic periodontitis. However, no studies have reported cases of early-onset periodontitis with VitD deficiency. This study reports a 5-year-old male patient with early-onset periodontitis, VitD deficiency and VitD receptor (VDR) mutation. The patient was treated with VitD and calcium, and received systematic periodontal treatment. During the 12-year treatment, the periodontal conditions of this patient were stable. Our in vitro study found that VitD could promote the expression of alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), bone morphogenetic protein 2 (BMP2), bone gamma-carboxyglutamate protein (BGLAP), and VDR in the early osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Meanwhile, VitD could downregulate mRNA expression levels of Interleukin-6 (IL-6), Interleukin-8 (IL-8), Interleukin-1β (IL-1β) and protein levels of IL-6 in the tumor necrosis factor-α (TNF-α) -induced inflammation of PDLSCs. Therefore, sufficient VitD supply can be a potential treatment for VitD deficiency induced early-onset periodontitis.

Keywords: 1,25(OH)(2)VitD(3); Early tooth loss; Osteogenic differentiation; Periodontal ligament stem cells; VitD receptor.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Aggressive Periodontitis / drug therapy
  • Aggressive Periodontitis / genetics
  • Aggressive Periodontitis / pathology
  • Animals
  • Bone Morphogenetic Protein 2 / genetics
  • Calcitriol / administration & dosage*
  • Cell Differentiation / drug effects*
  • Child
  • Child, Preschool
  • Core Binding Factor Alpha 1 Subunit / genetics
  • Humans
  • Inflammation / drug therapy
  • Inflammation / genetics
  • Male
  • Osteocalcin / genetics
  • Osteogenesis / drug effects*
  • Periodontal Ligament / drug effects
  • Periodontal Ligament / growth & development
  • Receptors, Calcitriol / genetics*
  • Stem Cells / drug effects
  • Tumor Necrosis Factor-alpha
  • Vitamin D / metabolism
  • Vitamin D Deficiency / drug therapy*
  • Vitamin D Deficiency / genetics
  • Vitamin D Deficiency / pathology

Substances

  • BGLAP protein, human
  • BMP2 protein, human
  • Bone Morphogenetic Protein 2
  • Core Binding Factor Alpha 1 Subunit
  • Receptors, Calcitriol
  • Tumor Necrosis Factor-alpha
  • VDR protein, human
  • Osteocalcin
  • Vitamin D
  • Calcitriol