Adaptive changes in individual acyl-CoA esters from hamster BAT during cold acclimation

Am J Physiol. 1988 Feb;254(2 Pt 1):E181-6. doi: 10.1152/ajpendo.1988.254.2.E181.

Abstract

Long-chain fatty acyl-CoA esters (LCFACoAE) were extracted from freeze-clamped powdered brown adipose tissue (BAT) obtained from thermoneutral control and cold-acclimated hamsters and the CoA esters individually separated by high-performance liquid chromatography. LCFACoAE of carbon chain length C12 to C20 were identified by increasing column retention time in the following order: C12:0, C14:1, C14:0, C16:1, C18:2, C16:0, C18:1, C18:0, and C20:4. The mean total LCFACoAE concentrations were 235 +/- 40 nmol/g protein for the control hamsters and 648 +/- 105 nmol/g protein for the 22-day cold-acclimated hamsters. A rapid fourfold increase in the levels of C16:0, C18:0, and C18:1 occurred within hours after initiation of the cold temperature, whereas the concentrations of the other six LCFACoAE either increased only slightly or remained unchanged. Almost 50% of the total LCFACoAE in the BAT of cold-acclimated hamsters was made up of C16:0, C18:0, and C18:1. These results, which demonstrate some dynamic changes in adipose tissue LCFACoAE, are consistent with their proposed role in the initiation and maintenance of BAT thermogenesis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acclimatization*
  • Acyl Coenzyme A / metabolism*
  • Adaptation, Physiological*
  • Adipose Tissue, Brown / metabolism*
  • Animals
  • Carboxylic Acids / metabolism*
  • Chromatography
  • Cold Temperature*
  • Cricetinae
  • Esters / metabolism*
  • Male
  • Mesocricetus
  • Osmolar Concentration
  • Time Factors

Substances

  • Acyl Coenzyme A
  • Carboxylic Acids
  • Esters