Pre-targeted immunoscintigraphy of murine tumors with indium-111-labeled bifunctional haptens

J Nucl Med. 1988 Feb;29(2):226-34.

Abstract

A method of tumor imaging utilizing the nonspecific accumulation of antibody through leaky capillaries is described, in which the antibody and the radiolabel are administered separately. Nonradioactive antibody is given first (pre-targeted), and allowed adequate time to reach maximum tumor concentration. Depending on the antibody, this may take several days. At the time of maximum tumor concentration of nonradioactive antibody, the blood is quickly cleared of excess circulating nonradioactive antibody using a special i.v. "chase". The radiolabel then is given and imaging done in 1 to 3 hr. The use of short lived tracers (hours) to image antibodies that localize slowly (days) in-vivo is made possible by this method.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • Haptens*
  • Indium Radioisotopes*
  • Methods
  • Mice
  • Neoplasms, Experimental / diagnostic imaging*
  • Neoplasms, Experimental / immunology
  • Radionuclide Imaging

Substances

  • Antibodies, Monoclonal
  • Haptens
  • Indium Radioisotopes