Noncatalytic protein component of elastase from horse leucocytes. A protein with regulatory function

J Potempa; E Korzus; J Silberring; A Dubin

doi:10.1016/0020-711x(88)90019-5

Noncatalytic protein component of elastase from horse leucocytes. A protein with regulatory function

Int J Biochem. 1988;20(1):105-8. doi: 10.1016/0020-711x(88)90019-5.

Authors

J Potempa¹, E Korzus, J Silberring, A Dubin

Affiliation

¹ Institute of Molecular Biology, Jagiellonian University, Cracow, Poland.

PMID: 3342921
DOI: 10.1016/0020-711x(88)90019-5

Abstract

1. Noncatalytic protein component (NPC), a strongly acidic protein (pH = 4.5) was separated from native horse leucocyte elastase 1. 2. This protein reduces elastinolytic properties of elastases: 1 and 2A probably by decreasing their isoelectric points. 3. A possible regulatory role of this protein may be inferred from a higher affinity of elastase 1 to NPC rather than to elastin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blood Proteins / physiology*
Electrophoresis, Polyacrylamide Gel
Horses
Immunoelectrophoresis, Two-Dimensional
Kinetics
Leukocytes / enzymology*
Pancreatic Elastase / blood*
Pancreatic Elastase / isolation & purification

Substances

Blood Proteins
Pancreatic Elastase