Identifying the effect of vancomycin on health care-associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media

Akanksha Rajput; Saugat Poudel; Hannah Tsunemoto; Michael Meehan; Richard Szubin; Connor A Olson; Yara Seif; Anne Lamsa; Nicholas Dillon; Alison Vrbanac; Joseph Sugie; Samira Dahesh; Jonathan M Monk; Pieter C Dorrestein; Rob Knight; Joe Pogliano; Victor Nizet; Adam M Feist; Bernhard O Palsson

doi:10.1093/gigascience/giaa156

Identifying the effect of vancomycin on health care-associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media

Gigascience. 2021 Jan 9;10(1):giaa156. doi: 10.1093/gigascience/giaa156.

Authors

Akanksha Rajput¹, Saugat Poudel¹, Hannah Tsunemoto², Michael Meehan^{3

4}, Richard Szubin¹, Connor A Olson¹, Yara Seif¹, Anne Lamsa², Nicholas Dillon^{5

6}, Alison Vrbanac^{5

6}, Joseph Sugie², Samira Dahesh^{5

6}, Jonathan M Monk¹, Pieter C Dorrestein^{3

4

7

8}, Rob Knight^{1

5

8

9}, Joe Pogliano², Victor Nizet^{4

5

6

8}, Adam M Feist^{1

10}, Bernhard O Palsson^{1

5

8

10}

Affiliations

¹ Department of Bioengineering, University of California, 9500 Gilman Dr, La Jolla, CA 92093, USA.
² Division of Biological Sciences, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
³ Collaborative Mass Spectrometry Innovation Center, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
⁴ Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
⁵ Department of Pediatrics, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92023, USA.
⁶ Collaborative to Halt Antibiotic-Resistant Microbes (CHARM), Department of Pediatrics, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
⁷ Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
⁸ Center for Microbiome Innovation, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
⁹ Department of Computer Science and Engineering, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA.
¹⁰ Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800 Kongens, Lyngby, Denmark.

Abstract

Background: The evolving antibiotic-resistant behavior of health care-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) USA100 strains are of major concern. They are resistant to a broad class of antibiotics such as macrolides, aminoglycosides, fluoroquinolones, and many more.

Findings: The selection of appropriate antibiotic susceptibility examination media is very important. Thus, we use bacteriological (cation-adjusted Mueller-Hinton broth) as well as physiological (R10LB) media to determine the effect of vancomycin on USA100 strains. The study includes the profiling behavior of HA-MRSA USA100 D592 and D712 strains in the presence of vancomycin through various high-throughput assays. The US100 D592 and D712 strains were characterized at sub-inhibitory concentrations through growth curves, RNA sequencing, bacterial cytological profiling, and exo-metabolomics high throughput experiments.

Conclusions: The study reveals the vancomycin resistance behavior of HA-MRSA USA100 strains in dual media conditions using wide-ranging experiments.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Delivery of Health Care
Humans
Methicillin-Resistant Staphylococcus aureus* / genetics
Microbial Sensitivity Tests
Staphylococcal Infections* / drug therapy
Vancomycin / pharmacology

Identifying the effect of vancomycin on health care-associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media

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