Warmer temperatures interact with salinity to weaken physiological facilitation to stress in freshwater fishes

Richard H Walker; Geoffrey D Smith; Spencer B Hudson; Susannah S French; Annika W Walters

doi:10.1093/conphys/coaa107

Warmer temperatures interact with salinity to weaken physiological facilitation to stress in freshwater fishes

Conserv Physiol. 2020 Dec 15;8(1):coaa107. doi: 10.1093/conphys/coaa107. eCollection 2020.

Authors

Richard H Walker^{1

2}, Geoffrey D Smith^{3

4}, Spencer B Hudson⁴, Susannah S French⁴, Annika W Walters^{5

2}

Affiliations

¹ Wyoming Cooperative Fish and Wildlife Research Unit, Department of Zoology and Physiology, University of Wyoming, 1000 E University Ave, Laramie, WY 82071, USA.
² Program in Ecology, University of Wyoming, 1000 E University Ave, Laramie, WY 82071, USA.
³ Biological Science Department, Dixie State University, 225 S 700 E, St. George, UT 84770, USA.
⁴ Department of Biology, Utah State University, 1435 Old Main Hill, Logan, UT 84322, USA.
⁵ US Geological Survey, Wyoming Cooperative Fish and Wildlife Research Unit, Department of Zoology and Physiology, University of Wyoming, 1000 E University Ave, Laramie, WY 82071, USA.

Abstract

Management of stressors requires an understanding of how multiple stressors interact, how different species respond to those interactions and the underlying mechanisms driving observed patterns in species' responses. Salinization and rising temperatures are two pertinent stressors predicted to intensify in freshwater ecosystems, posing concern for how susceptible organisms achieve and maintain homeostasis (i.e. allostasis). Here, glucocorticoid hormones (e.g. cortisol), responsible for mobilizing energy (e.g. glucose) to relevant physiological processes for the duration of stressors, are liable to vary in response to the duration and severity of salinization and temperature rises. With field and laboratory studies, we evaluated how both salinity and temperature influence basal and stress-reactive cortisol and glucose levels in age 1+ mottled sculpin (Cottus bairdii), mountain sucker (Catostomus platyrhynchus) and Colorado River cutthroat trout (Oncorhynchus clarki pleuriticus). We found that temperature generally had the greatest effect on cortisol and glucose concentrations and the effect of salinity was often temperature dependent. We also found that when individuals were chronically exposed to higher salinities, baseline concentrations of cortisol and glucose usually declined as salinity increased. Reductions in baseline concentrations facilitated stronger stress reactivity for cortisol and glucose when exposed to additional stressors, which weakened as temperatures increased. Controlled temperatures near the species' thermal maxima became the overriding factor regulating fish physiology, resulting in inhibitory responses. With projected increases in freshwater salinization and temperatures, efforts to reduce the negative effects of increasing temperatures (i.e. increased refuge habitats and riparian cover) could moderate the inhibitory effects of temperature-dependent effects of salinization for freshwater fishes.

Keywords: Antagonistic interaction; cortisol; glucose; multiple stressors; sodium bicarbonate; stress reactivity.