Enhanced production of methane in anaerobic water treatment as mediated by the immobilized fungi

Abstract

Anaerobic digestion of organic waste and wastewater represents an attractive sustainable bio-technology to produce methane as an alternative to fossil energy. In response to improvement of methane production via enhancing methanogenesis, current strategies of the addition of external biological/non-biological materials have to confront either the loss of materials, high cost and/or possible destruction of the microbial community. Here, we report the first case of using immobilized fungi Aspergillus sydowii 8L-9-F02 to optimize the microbial community, achieving remarkable improvement of the methane production in both batch test (1.5 times) and continuous flow operation (1.13-1.31 times). The crucial role of fungi is associated with the stimulation of enrichment of Methanosaeta and Methanobacterium for methanogenesis from 28.2 to 67.4% as well as the improved activity of enzyme F420. Moreover, fungi also increase the content of extracellular polymeric substances, facilitating the formation of bio-aggregates. This work provides a new pathway to enhance methanogenesis during anaerobic digestion of wastewater by using fungi as bio-enhancer.

Keywords: Anaerobic digestion; Fungi; Immobilization; Methanogenesis; Wastewater.