Identification of Bioactive Components from Ruellia tuberosa L. on Improving Glucose Uptake in TNF- α-Induced Insulin-Resistant Mouse FL83B Hepatocytes

Jian-Hua Xu; Yangming Martin Lo; Wen-Chang Chang; Da-Wei Huang; James Swi-Bea Wu; Yu-Yuan Jhang; Wen-Chung Huang; Chih-Yuan Ko; Szu-Chuan Shen

doi:10.1155/2020/6644253

Identification of Bioactive Components from Ruellia tuberosa L. on Improving Glucose Uptake in TNF- α-Induced Insulin-Resistant Mouse FL83B Hepatocytes

Evid Based Complement Alternat Med. 2020 Dec 1:2020:6644253. doi: 10.1155/2020/6644253. eCollection 2020.

Authors

Jian-Hua Xu¹, Yangming Martin Lo², Wen-Chang Chang³, Da-Wei Huang⁴, James Swi-Bea Wu⁵, Yu-Yuan Jhang⁶, Wen-Chung Huang⁷, Chih-Yuan Ko^{8

9

10

11}, Szu-Chuan Shen⁶

Affiliations

¹ Department of Tumor Surgery, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China.
² Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China.
³ Department of Food Science, National Chiayi University, Chiayi City 60004, Taiwan.
⁴ Department of Biotechnology and Food Technology, Southern Taiwan University of Science and Technology, Tainan City 71005, Taiwan.
⁵ Graduate Institute of Food Science and Technology, National Taiwan University, Taipei 10672, Taiwan.
⁶ Graduate Program of Nutrition Science, National Taiwan Normal University, Taipei 10610, Taiwan.
⁷ Graduate Institute of Health Industry Technology, Chang Gung University of Science and Technology, Taoyuan 33303, Taiwan.
⁸ Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China.
⁹ Department of Clinical Nutrition, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China.
¹⁰ School of Public Health, Fujian Medical University, Fuzhou 350122, Fujian, China.
¹¹ Respiratory Medicine Center of Fujian Province, Quanzhou 362000, China.

Abstract

Ruellia tuberosa L. (RTL) has been used as a folk medicine to cure diabetes in Asia. RTL was previously reported to alleviate hyperglycemia, insulin resistance (IR), abnormal hepatic detoxification, and liver steatosis. However, the potential bioactive compounds of RTL have still not been identified. The aim of this study was to investigate the bioactive compounds in RTL ethyl acetate (EA) fractions by using a glucose uptake assay in TNF-α-treated mouse FL83B hepatocytes to discover a mechanism by which to improve IR. The bioactive compounds were identified by the high-performance liquid chromatography (HPLC) assay. Using the Sephadex LH20 gel packing chromatography column, the EAF5 fraction was isolated from RTL and significantly increased glucose uptake in TNF-α-treated FL83B cells. Moreover, the MCI gel packing chromatography column separated EAF5 into five subfractions and had no significant cytotoxic effect in FL83B cells when treated at the concentration of 25 μg/ml. Among the subfractions, EAF5-5 markedly enhanced glucose uptake in TNF-α-treated FL83B cells. The possible bioactive compounds of the EAF5-5 fraction that were identified by the HPLC assay include syringic acid, p-coumaric acid, and cirsimaritin. The bioactive compound with the best effect of increasing glucose uptake was p-coumaric acid, but its effect alone was not as good as the combined effect of all three compounds of the EAF5-5 fraction. Thus, we speculate that the antidiabetic effect of RTL may be the result of multiple active ingredients.