Absence of Integrase Inhibitor-Associated Resistance Among Antiretroviral Therapy-Naïve HIV-1-Infected Adults in Guangdong Province, China, in 2018

Yun Lan; Linghua Li; Weilie Chen; Xizi Deng; Junbin Li; Qinghong Fan; Xiaoli Cai; Weiping Cai; Fengyu Hu

doi:10.2147/IDR.S284917

Absence of Integrase Inhibitor-Associated Resistance Among Antiretroviral Therapy-Naïve HIV-1-Infected Adults in Guangdong Province, China, in 2018

Infect Drug Resist. 2020 Dec 8:13:4389-4394. doi: 10.2147/IDR.S284917. eCollection 2020.

Authors

Yun Lan^#¹, Linghua Li^#², Weilie Chen^#¹, Xizi Deng¹, Junbin Li², Qinghong Fan¹, Xiaoli Cai¹, Weiping Cai², Fengyu Hu¹

Affiliations

¹ Institute of Infectious Disease, Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong, People's Republic of China.
² Infectious Disease Center, Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong, People's Republic of China.

^# Contributed equally.

Abstract

Background: Antiretroviral therapy (ART) containing an integrase strand transfer inhibitor (INSTI) plus two nucleoside reverse-transcriptase inhibitors has been recommended as a first-line regimen for ART-naïve HIV-1-infected patients in the latest Chinese Guidelines for Diagnosis and Treatment of HIV/AIDS.

Objective: To determine the prevalence of INSTI-related mutations among ART-naïve HIV-1-infected adults in Guangdong, China, in 2018.

Methods: The entire integrase gene was amplified from blood plasma. Demographic and epidemiological information was collected. INSTI mutations and antiretroviral susceptibility were interpreted using the Stanford University HIV Drug Resistance Database HIVdb program.

Results: Of 927 samples, 827 integrase sequences were successfully obtained. Among them, no major resistance mutations to INSTIs were identified, and four accessory mutations, including T97A (0.12%, 1/827), A128T (0.24%, 2/827), E157Q (0.85%, 7/827), and G163R (0.24%, 2/827), were found in twelve individuals. Two patient samples contained the G163R mutation conferring low-level resistance to elvitegravir and raltegravir.

Conclusion: The overall prevalence of INSTI mutations remains low. Drug resistance mutation testing for the detection of INSTI drug resistance mutations in HIV treatment-naïve patients should be considered due to the circulation of polymorphisms contributing to INSTI resistance and the expected increasing use of this class of drugs.

Keywords: Guangdong; HIV-1; drug resistance mutation; integrase inhibitor.

Grants and funding

This study was funded by the Guangzhou Science and Technology Plan Project (202002030028), and the National Major Scientific and Technological Project (2018ZX10302103-002, 2017ZX10202101-003 and 2018ZX10732101-001-014).