The RAD50 gene of Saccharomyces cerevisiae is not essential for vegetative growth

M Kupiec

doi:10.1007/BF00419878

The RAD50 gene of Saccharomyces cerevisiae is not essential for vegetative growth

Curr Genet. 1986;10(6):487-9. doi: 10.1007/BF00419878.

Author

M Kupiec¹

Affiliation

¹ Department of Genetics, Hebrew University, Jerusalem, Israel.

PMID: 3327607
DOI: 10.1007/BF00419878

Abstract

The gene RAD50 was located by the ability of subclones to restore the Rad+ phenotype following transformation of a rad50-1 mutant. Disruption of the gene was achieved by directed integration of a plasmid carrying a fragment internal to RAD50. Haploids with the disrupted gene are viable and do not differ in growth rate or plating efficiency from isogenic rad50-1 or Rad+ strains.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Cloning, Molecular
DNA Mutational Analysis
DNA Repair*
Genes, Fungal*
Recombination, Genetic
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / growth & development