Effects of biochar on biodegradation of sulfamethoxazole and chloramphenicol by Pseudomonas stutzeri and Shewanella putrefaciens: Microbial growth, fatty acids, and the expression quantity of genes

Abstract

An incubation experiment was conducted to investigate whether different biochar could enhance the biodegradation of sulfamethoxazole (SMX) and chloramphenicol (CAP). During incubation in nutrient medium solution, the degradation efficiencies of SMX by P. stutzeri and S. putrefaciens obtained 61.79% and 68.67% respectively, while CAP was 85.75% and 85.70%. The biodegradation efficiencies of SMX and CAP increased for P. stutzeri cultured with biochar and increased for S. putrefaciens cultured with high-concentration biochar (500, 1,000, 2,000 mg L^-1). Additionally, TOC and TN contents were significantly decreased during the biodegradation process. Hence, the effects of biochar on microbial growth, fatty acids and expression genes, biodegradation products were studied. The content of bacteria, saturated fatty acids and expression genes showed a positive correlation with the content of TOC released from biochar, while the biodegradation products would not change when bacteria was cultured with biochar. These indicated that biochar improved the antibiotics biodegradation efficiencies via involvement in the bacterial growth, changing the components of fatty acids, increasing the expression quantity of genes. This research suggests that micro-biological degradation with biochar is a promising technology to treat specific antibiotics in the environment.

Keywords: Antibiotics; Biochar; Biodegradation; Pseudomonas stutzeri; Shewanella putrefaciens.