Histologic effects of MicroPulse™ transscleral cyclophotocoagulation in normal equine eyes

Vet Ophthalmol. 2021 Jan;24(1):59-70. doi: 10.1111/vop.12846. Epub 2020 Nov 29.

Abstract

Objective: Determine the immediate post-operative effects of MicroPulse transscleral cyclophotocoagulation (MP-TSCPC) in healthy equine eyes.

Animals studied: Ten adult horses.

Methods: MP-TSCPC was performed on sedated horses in 12 eyes (4 groups) using the following parameters (power, duration, duty cycle): (1) 2000 mW, 180 seconds, 31.3%; (2) 3000 mW, 180 seconds, 31.3%; (3) 3000 mW, 270 seconds, 31.3%; and (4) 3000 mW, 270 seconds, 50%. Three additional eyes were left untreated (control). Eyes were monitored clinically until euthanasia (mean = 3 hours post-procedure). Histologic sections were assessed with light microscopy and transmission electron microscopy (TEM).

Results: MP-TSCPC was well tolerated by sedated horses. Adverse effects were only noted in Group 4: ocular hypertension (n = 3/3), conjunctival burns (3/3), aqueous flare (2/3), and a corneal erosion (1/3). Histologic scoring of Group 4 was statistically greater than other treated groups (1-3) and control eyes (P ≤ .021). TEM showed subtle changes to the mitochondria and plasma membrane infoldings of the basilar surface of the nonpigmented epithelium in all treated eyes.

Conclusions: MP-TSCPC does not cause immediate post-procedure adverse clinical effects or pronounced morphological changes to the ciliary body, except with the highest laser settings evaluated (power 3000 mW, duration 270 seconds, duty cycle 50%).

Keywords: ciliary body; equine; glaucoma; micropulse; ocular histopathology; transmission electron microscopy.

Publication types

  • Clinical Trial, Veterinary

MeSH terms

  • Animals
  • Ciliary Body / anatomy & histology
  • Ciliary Body / surgery
  • Ciliary Body / ultrastructure
  • Eye / anatomy & histology*
  • Eye / ultrastructure
  • Female
  • Horses / surgery*
  • Laser Coagulation / methods
  • Laser Coagulation / veterinary*
  • Male
  • Postoperative Period