Chromatin Tracing: Imaging 3D Genome and Nucleome

Mengwei Hu; Siyuan Wang

doi:10.1016/j.tcb.2020.10.006

Chromatin Tracing: Imaging 3D Genome and Nucleome

Trends Cell Biol. 2021 Jan;31(1):5-8. doi: 10.1016/j.tcb.2020.10.006. Epub 2020 Nov 12.

Authors

Mengwei Hu¹, Siyuan Wang²

Affiliations

¹ Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.
² Department of Genetics, Yale University School of Medicine, New Haven, CT, USA; Department of Cell Biology, Yale University School of Medicine, New Haven, CT, USA; Yale Combined Program in the Biological and Biomedical Sciences, Yale University, New Haven, CT, USA; Molecular Cell Biology, Genetics, and Development Program, Yale University, New Haven, CT, USA; Biochemistry, Quantitative Biology, Biophysics, and Structural Biology Program, Yale University, New Haven, CT, USA; MD-PhD Program, Yale University, New Haven, CT, USA; Yale Center for RNA Science and Medicine, Yale University School of Medicine, New Haven, CT, USA; Yale Liver Center, Yale University School of Medicine, New Haven, CT, USA. Electronic address: siyuan.wang@yale.edu.

Abstract

Correct 3D genome organization is essential for the proper functioning of the genome. Recent advances in image-based 3D genomics techniques have enabled direct tracing of chromatin folding and multiplexed imaging of nucleome architectures in single cells of several important biological systems. Here, we discuss these advances and the future directions of image-based 3D genomics.

Keywords: 3D genome; chromatin folding; chromatin tracing; image-based spatial omics; multiplexed imaging of nucleome architectures; multiplexed sequential fluorescent in situ hybridization.

MeSH terms

Animals
Chromatin / metabolism*
Genome*
Genomics
Humans
Imaging, Three-Dimensional*
Transcriptome / genetics

Substances

Chromatin

Abstract

MeSH terms

Substances

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