Rapid multiplex PCR assay for simultaneous detection of Neisseria gonorrhoeae and Chlamydia trachomatis in genitourinary samples: A 30-minute assay

J Microbiol Methods. 2021 Jan:180:106103. doi: 10.1016/j.mimet.2020.106103. Epub 2020 Nov 12.

Abstract

We developed a rapid multiplex PCR assay available in bedside for the simultaneous detection of Neisseria gonorrhoeae and Chlamydia trachomatis, which enables diagnosis in less than 30 minutes. In this study, we validated the clinical utility of this assay including its sensitivity and specificity for NG and CT detection.

Keywords: C. trachomatis; N. gonorrhoeae; Nucleic acid amplification tests; Rapid multiplex PCR; Sexually transmitted infections.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Chlamydia Infections / diagnosis*
  • Chlamydia Infections / microbiology
  • Chlamydia Infections / urine
  • Chlamydia trachomatis / genetics*
  • Chlamydia trachomatis / isolation & purification*
  • DNA, Bacterial / analysis
  • Female
  • Gonorrhea / diagnosis*
  • Gonorrhea / microbiology
  • Gonorrhea / urine
  • Humans
  • Male
  • Multiplex Polymerase Chain Reaction / methods*
  • Neisseria gonorrhoeae / genetics*
  • Neisseria gonorrhoeae / isolation & purification*
  • Sensitivity and Specificity
  • Sexually Transmitted Diseases / diagnosis
  • Sexually Transmitted Diseases / microbiology

Substances

  • DNA, Bacterial