A small-sized benzothiazole-indolium fluorescent probe: the study of interaction specificity targeting c-MYC promoter G-quadruplex structures and live cell imaging

Chem Commun (Camb). 2020 Dec 7;56(95):15016-15019. doi: 10.1039/d0cc06525k.

Abstract

A small-sized c-MYC promoter G-quadruplex selective fluorescent BZT-Indolium binding ligand was demonstrated for the first time as a highly target-specific and photostable probe for in vitro staining and live cell imaging and it was found to be able to inhibit the amplification of the c-MYC G-rich sequence (G-quadruplex) and down-regulate oncogene c-MYC expression in human cancer cells (HeLa).

MeSH terms

  • Amino Acid Sequence
  • Benzothiazoles / chemistry*
  • Biosensing Techniques
  • DNA-Binding Proteins / analysis*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Down-Regulation
  • Fluorescent Dyes / chemistry*
  • G-Quadruplexes
  • HeLa Cells
  • Humans
  • Indoles / chemistry*
  • Ligands
  • Optical Imaging
  • Promoter Regions, Genetic
  • Sensitivity and Specificity
  • Staining and Labeling
  • Transcription Factors / analysis*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • Benzothiazoles
  • DNA-Binding Proteins
  • Fluorescent Dyes
  • Indoles
  • Ligands
  • MYCBP protein, human
  • Transcription Factors