Structural characterization of enzymatic modification of Hericium erinaceus polysaccharide and its immune-enhancement activity

Int J Biol Macromol. 2021 Jan 1:166:1396-1408. doi: 10.1016/j.ijbiomac.2020.11.019. Epub 2020 Nov 6.

Abstract

In this study, the enzyme degradation of Hericium erinaceus polysaccharide (HEP) was successfully modified with endo-rhamnosidase to obtain the enzymatic hydrolysis of Hericium erinaceus polysaccharide product (EHEP). The gas chromatography-mass spectrometry (GC-MS), high performance gel permeation chromatography (HPGPC), Fourier transformed infrared spectrometry (FT-IR), scanning electron microscopy (SEM), atomic particle microscopy (AFM), nuclear magnetic resonance (NMR) and particle size distribution were used to characterize polysaccharides. In vitro, EHEP significantly enhanced the phagocytosis, NO, CD40 and CD86 by macrophage than HEP. In vivo, female Balb/c mice were injected respectively with EHEP and HEP after administrated with cyclophosphamide, once a day for 7 days. On days 11, the morphology and structure of jejunal sections, immunofluorescence of spleen and peritoneal macrophages were determined. These results indicated that the enzymatic hydrolysis product could enhance the activation of peritoneal macrophages, and enhance the immunomodulation function of HEP. This study demonstrated that enzymatic modification was an effective method to improve the activities of HEP, and could be developed as a potential technology for use in pharmaceutical and cosmeceutical industry.

Keywords: Enzymatic hydrolysis; Hericium erinaceus polysaccharide; Immunomodulatory activities.

MeSH terms

  • Animals
  • B7-2 Antigen / genetics
  • B7-2 Antigen / metabolism
  • CD40 Antigens / genetics
  • CD40 Antigens / metabolism
  • Female
  • Fungal Polysaccharides / chemistry*
  • Fungal Polysaccharides / pharmacology
  • Hericium / chemistry*
  • Immunologic Factors / chemistry*
  • Immunologic Factors / pharmacology
  • Macrophage Activation*
  • Macrophages, Peritoneal / drug effects
  • Macrophages, Peritoneal / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Nitric Oxide / metabolism
  • Phagocytosis
  • RAW 264.7 Cells
  • Spleen / drug effects
  • Spleen / metabolism

Substances

  • B7-2 Antigen
  • CD40 Antigens
  • Fungal Polysaccharides
  • Immunologic Factors
  • Nitric Oxide

Supplementary concepts

  • Hericium erinaceus