Maintaining Context in Ice: Cryo-EM/ET Workflow Optimizations

Nadav Elad; Sharon Grayer Wolf

doi:10.1016/j.str.2020.10.004

Maintaining Context in Ice: Cryo-EM/ET Workflow Optimizations

Structure. 2020 Nov 3;28(11):1179-1181. doi: 10.1016/j.str.2020.10.004.

Authors

Nadav Elad¹, Sharon Grayer Wolf²

Affiliations

¹ Department of Chemical Research Support, Weizmann Institute of Science, Rehovot, Israel. Electronic address: nadav.elad@weizmann.ac.il.
² Department of Chemical Research Support, Weizmann Institute of Science, Rehovot, Israel. Electronic address: sharon.wolf@weizmann.ac.il.

PMID: 33147474
DOI: 10.1016/j.str.2020.10.004

Abstract

In this issue of Structure, breakthroughs in cryo-EM/ET research are presented. Klebl et al. (2020) demonstrate how speed in sample vitrification impacts the quality of macromolecular particles in resultant cryo-EM grids. Wu et al. (2020) combine fluorescence, ion beam milling, and tomography to unravel unique features in vitrified yeast cells.

Publication types

Comment

MeSH terms

Cryoelectron Microscopy*
Electron Microscope Tomography*
Ice
Macromolecular Substances
Workflow

Substances

Ice
Macromolecular Substances