Dynamic Clamp on a Windows PC

Lorin S Milescu; Joël Tabak

doi:10.1007/978-1-0716-0818-0_8

Dynamic Clamp on a Windows PC

Methods Mol Biol. 2021:2188:157-177. doi: 10.1007/978-1-0716-0818-0_8.

Authors

Lorin S Milescu¹, Joël Tabak²

Affiliations

¹ Department of Biology, University of Maryland at College Park, College Park, MD, USA. lorinsmilescu@gmail.com.
² Institute of Biomedical and Clinical Science, University of Exeter Medical School, Exeter, Devon, UK. j.tabak@exeter.ac.uk.

PMID: 33119851
DOI: 10.1007/978-1-0716-0818-0_8

Abstract

Dynamic clamp is a powerful tool for interfacing computational models and real cells. We describe here how to set up and carry out dynamic clamp experiments using a patch clamp amplifier, a National Instruments data acquisition card, and the freely available QuB software that operates on a PC running MS Windows.

Keywords: Action potential; Dynamic clamp; Ion channels; Patch clamp; QuB; Real-time computation.

MeSH terms

Action Potentials
Animals
Computer Simulation
Electrophysiology
Humans
Ion Channels / metabolism
Models, Neurological
Neurons / metabolism
Patch-Clamp Techniques / methods*
Software*

Substances

Ion Channels