Red emission ratio fluorescent probe for the activity of vanin-1 and imaging in vivo

Jing Qian; Liang Zhang; Jiemin Wang; Zhidong Teng; Ting Cao; Lei Zheng; Yuping Cao; Wenwu Qin; Yun Liu; Huichen Guo

doi:10.1016/j.jhazmat.2020.123863

Red emission ratio fluorescent probe for the activity of vanin-1 and imaging in vivo

J Hazard Mater. 2021 Jan 5:401:123863. doi: 10.1016/j.jhazmat.2020.123863. Epub 2020 Sep 2.

Authors

Jing Qian¹, Liang Zhang², Jiemin Wang¹, Zhidong Teng³, Ting Cao¹, Lei Zheng¹, Yuping Cao¹, Wenwu Qin⁴, Yun Liu⁵, Huichen Guo⁶

Affiliations

¹ State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and Key Laboratory of Special Function Materials and Structure Design (MOE), College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
² Department of Radiology, Xinqiao Hospital, Army Medical University, Chongqing, 400037, PR China.
³ State Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu Province 730046, PR China.
⁴ State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and Key Laboratory of Special Function Materials and Structure Design (MOE), College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China. Electronic address: qinww@lzu.edu.cn.
⁵ Department of Radiology, Xinqiao Hospital, Army Medical University, Chongqing, 400037, PR China. Electronic address: yunliu@tmmu.edu.cn.
⁶ State Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu Province 730046, PR China. Electronic address: guohuichen@caas.cn.

PMID: 33113750
DOI: 10.1016/j.jhazmat.2020.123863

Abstract

Pantetheinase, also known as Vanin-1, catalyzes pantetheine to decompose into the precursor of CoA - pantothenic acid and aminothiol cysteamine. Studies have shown that Vanin-1 plays an important role in many important physiological pathologies. In this paper, a new red emission ratio fluorescent probe DCM-PA (I_{640 nm}/I_{564 nm}) has been implemented to detect the activity of Vanin-1 in cells and vivo. DCM-PA has short response time (30 min), high selectivity and low sensitivity (DL =0.69 ng/mL). Also, we have applied DCM-PA for imaging in cells and mice, and the results have indicated that the probe has a non-negligible potential for monitoring the activity of Vanin-1 in situ, benefiting further to study the role of Vanin-1 in physiology and pathology. In addition, the up-regulation of this enzyme by starvation confirmed the inevitable connection between diabetes and abnormal expression of Vanin-1.

Keywords: Diabetes; Imaging in vivo; Red emission; Starvation-induced; Vanin-1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cysteamine
Fluorescent Dyes*
Mice
Pantothenic Acid* / metabolism
Up-Regulation

Substances

Fluorescent Dyes
Pantothenic Acid
Cysteamine