Protocol to Prepare Single-Cell Suspensions from Mouse Vagal Sensory Ganglia for Transcriptomic Studies

Martin Häring; Michael Fatt; Jussi Kupari

doi:10.1016/j.xpro.2020.100030

Protocol to Prepare Single-Cell Suspensions from Mouse Vagal Sensory Ganglia for Transcriptomic Studies

STAR Protoc. 2020 Jun 3;1(1):100030. doi: 10.1016/j.xpro.2020.100030. eCollection 2020 Jun 19.

Authors

Martin Häring^{1

2}, Michael Fatt¹, Jussi Kupari¹

Affiliations

¹ Division of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 17177 Stockholm, Sweden.
² Department of Molecular Neurosciences, Center for Brain Research, Medical University Vienna, 1090 Vienna, Austria.

Abstract

Vagal sensory neurons relay viscero- and somatosensory information from within the body and play a key role in maintaining physiological homeostasis. We recently characterized the diversity of vagal sensory neurons in the mouse using a single-cell transcriptomics approach. Here, we provide an in-depth protocol for the extraction of mouse vagal ganglia and the production of high-quality single-cell suspensions from this tissue. This effective protocol can also be applied for use with other peripheral and central neuron populations with few modifications. For complete details on the use and execution of this protocol, please refer to Kupari et al. (2019).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Culture Techniques / methods*
Cells, Cultured
Gene Expression Profiling / methods*
Mice
Sensory Receptor Cells / cytology*
Sensory Receptor Cells / metabolism
Single-Cell Analysis / methods*
Transcriptome
Vagus Nerve / cytology*
Vagus Nerve / metabolism