Panton-Valentine leukocidin (PVL) isoforms among Staphylococcus aureus lineages isolated from hospitals in Rio de Janeiro, Brazil

Braz J Microbiol. 2020 Dec;51(4):1509-1513. doi: 10.1007/s42770-020-00386-y. Epub 2020 Oct 26.

Abstract

Panton-Valentine leukocidin (PVL) is a Staphylococcus aureus virulence factor codified by lukSF-PV genes. Single-nucleotide polymorphisms (SNPs) at lukSF-PV genes can lead to two PVL sequence variants (R and H) generating different PVL isoforms. This study analyzed lukSF-PV genes SNPs among four different clonal lineages (STs/CC 1, 5, 8, and 30) of nine S. aureus isolated at Brazilian hospitals. The sequenced products showed SNPs at seven sites (positions 121, 470, 527, 663, 856, 1396, and 1729), leading to non-synonymous substitutions in all isolates investigated. Our findings showed new R and H isoforms variants in S. aureus isolated in Brazil and suggest a possible relationship between H2b isoform and the ST30/CC30 lineage.

Keywords: Brazilian lineages; Panton-Valentine leukocidin (PVL) isoforms; Staphylococcus aureus.

MeSH terms

  • Bacterial Toxins / genetics*
  • Brazil
  • Exotoxins / genetics*
  • Humans
  • Leukocidins / genetics*
  • Polymorphism, Single Nucleotide
  • Protein Isoforms
  • Staphylococcal Infections / microbiology*
  • Staphylococcus aureus / genetics*
  • Staphylococcus aureus / isolation & purification
  • Virulence Factors / genetics*

Substances

  • Bacterial Toxins
  • Exotoxins
  • Leukocidins
  • Panton-Valentine leukocidin
  • Protein Isoforms
  • Virulence Factors