Long-Range Interhemispheric Projection Neurons Show Biased Response Properties and Fine-Scale Local Subnetworks in Mouse Visual Cortex

Kenta M Hagihara; Ayako Wendy Ishikawa; Yumiko Yoshimura; Yoshiaki Tagawa; Kenichi Ohki

doi:10.1093/cercor/bhaa297

Long-Range Interhemispheric Projection Neurons Show Biased Response Properties and Fine-Scale Local Subnetworks in Mouse Visual Cortex

Cereb Cortex. 2021 Jan 5;31(2):1307-1315. doi: 10.1093/cercor/bhaa297.

Authors

Kenta M Hagihara^{1

2}, Ayako Wendy Ishikawa^{3

4

5}, Yumiko Yoshimura^{3

4}, Yoshiaki Tagawa^{6

7

8}, Kenichi Ohki^{1

8

9

10

11}

Affiliations

¹ Department of Molecular Physiology, Kyushu University Graduate School of Medical Sciences, Fukuoka 812-8582, Japan.
² Friedrich Miescher Institute for Biomedical Research, Basel 4058, Switzerland.
³ Division of Visual Information Processing, National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki 444-8585, Japan.
⁴ Department of Physiological Sciences, The Graduate University for Advanced Studies, Okazaki 444-8585, Japan.
⁵ Keio University School of Medicine, Shinanomachi, Shinjuku-ku, 160-8582, Japan.
⁶ Department of Biophysics, Kyoto University Graduate School of Science, Kyoto 606-8502, Japan.
⁷ Department of Physiology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan.
⁸ CREST, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan.
⁹ Department of Physiology, The University of Tokyo School of Medicine, Tokyo 113-0033, Japan.
¹⁰ International Research Center for Neurointelligence (WPI-IRCN), The University of Tokyo School of Medicine, Tokyo 113-0033, Japan.
¹¹ Institute for AI and Beyond, The University of Tokyo School of Medicine, Tokyo 113-0033, Japan.

PMID: 33063102
DOI: 10.1093/cercor/bhaa297

Abstract

Integration of information processed separately in distributed brain regions is essential for brain functions. This integration is enabled by long-range projection neurons, and further, concerted interactions between long-range projections and local microcircuits are crucial. It is not well known, however, how this interaction is implemented in cortical circuits. Here, to decipher this logic, using callosal projection neurons (CPNs) in layer 2/3 of the mouse visual cortex as a model of long-range projections, we found that CPNs exhibited distinct response properties and fine-scale local connectivity patterns. In vivo 2-photon calcium imaging revealed that CPNs showed a higher ipsilateral (to their somata) eye preference, and that CPN pairs showed stronger signal/noise correlation than random pairs. Slice recordings showed CPNs were preferentially connected to CPNs, demonstrating the existence of projection target-dependent fine-scale subnetworks. Collectively, our results suggest that long-range projection target predicts response properties and local connectivity of cortical projection neurons.

Keywords: 2-photon imaging; callosal projection neuron; ocular dominance; slice physiology; visual cortex.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Female
Male
Mice
Mice, Inbred C57BL
Nerve Net / chemistry
Nerve Net / physiology*
Neurons / chemistry
Neurons / physiology*
Organ Culture Techniques
Photic Stimulation / methods*
Visual Cortex / chemistry
Visual Cortex / physiology*
Visual Pathways / chemistry
Visual Pathways / physiology*