An electrospun nerve wrap comprising Bletilla striata polysaccharide with dual function for nerve regeneration and scar prevention

Shih-Heng Chen; Pang-Yun Chou; Zhi-Yu Chen; David Chwei-Chin Chuang; Sung-Tsang Hsieh; Feng-Huei Lin

doi:10.1016/j.carbpol.2020.116981

An electrospun nerve wrap comprising Bletilla striata polysaccharide with dual function for nerve regeneration and scar prevention

Carbohydr Polym. 2020 Dec 15:250:116981. doi: 10.1016/j.carbpol.2020.116981. Epub 2020 Aug 25.

Authors

Shih-Heng Chen¹, Pang-Yun Chou², Zhi-Yu Chen³, David Chwei-Chin Chuang², Sung-Tsang Hsieh⁴, Feng-Huei Lin⁵

Affiliations

¹ Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan; Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Chang Gung University and Medical College, Taoyuan, Taiwan.
² Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Chang Gung University and Medical College, Taoyuan, Taiwan.
³ Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan; Division of Biomedical Engineering and Nanomedicine Research, National Health Research Institutes, Miaoli, Taiwan.
⁴ Department of Anatomy and Cell Biology, National Taiwan University College of Medicine, Taiwan; Department of Neurology, National Taiwan University Hospital, Taiwan.
⁵ Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan; Division of Biomedical Engineering and Nanomedicine Research, National Health Research Institutes, Miaoli, Taiwan. Electronic address: double@ntu.edu.tw.

PMID: 33049868
DOI: 10.1016/j.carbpol.2020.116981

Abstract

Peripheral nerve injuries mainly occur in the extremities and result in functional deficits, and recovery after microsurgical repair is slow (<1 mm/day) and frustrating. To improve the results of nerve repair, it is crucial to promote local Schwann cell (SC) function, neurite outgrowth, and to reduce perineural adhesion. In this study, a nerve wrap electrospun with Bletilla striata polysaccharide (BSP) and waterborne polyurethane (WPU) was designed to wrap around the repaired nerve to achieve the goal. In SC proliferation/migration tests and neurite outgrowth assay, BSP/WPU revealed considerable improvement. In rabbit sciatic nerve repair model, BSP/WPU exhibited significantly higher compound muscle action potential, muscle contraction force, and reduced perineural adhesion. The downstream fascicles of the BSP/WPU group exhibited a 50 % increase in diameter and 100 % increase in myelin thickness compared with the control group. In vitro and in vivo experiments indicated the positive effect of BSP/WPU nerve wrap on nerve regeneration.

Keywords: Antiadhesion; Axon regeneration; Bletilla striata polysaccharide; Peripheral nerve regeneration; Schwann cell; Waterborne polyurethane.

MeSH terms

Animals
Cicatrix / prevention & control*
Electricity
Male
Nanofibers / chemistry*
Nerve Regeneration*
Orchidaceae / chemistry*
Polysaccharides / pharmacology*
Rabbits
Rats
Rats, Sprague-Dawley
Sciatic Nerve / cytology*

Substances

Polysaccharides