H2S pretreatment mitigates the alkaline salt stress on Malus hupehensis roots by regulating Na+/K+ homeostasis and oxidative stress

Abstract

Hydrogen sulfide (H₂S) plays an important role in the plant salt stress response. The main component of salt stress is neutral salt (NaCl); NaHCO₃ and Na₂CO₃ play a key role in soil alkaline due to the influence of pH. Malus hupehensis Rehd. var. pingyiensis Jiang (Pingyi Tiancha, PYTC) is a salt-sensitive apple rootstock. Seedlings of PYTC pretreated with NaHS (an H₂S donor) were exposed to an alkaline salt solution, and then the plant growth, root architecture, oxidative damage, Na⁺/K⁺ homeostasis and gene expression of MhSOS1 and MhSKOR were investigated. The results showed that NaHS pretreatment increased the endogenous H₂S content in seedlings, significantly alleviated the alkaline salt stress-induced growth inhibition and oxidative damage by inducing antioxidant enzymes activities, and sustained the root activity and root architecture of PYTC in the alkaline salt solution. NaHS pretreatment significantly decreased the root Na⁺ content and increased K⁺ content to maintain the homeostasis of Na⁺/K⁺, and effect the expression of MhSOS1 and MhSKOR at the transcription level in the presence of the alkaline salt. Our study reveals that application of H₂S could mitigate the toxic effect of alkaline salt stress on Malus hupehensis seedlings, thus providing a foundation for improved plant tolerance to alkaline salt stress.

Keywords: Alkaline salt; Hydrogen sulfide; Malus hupehensis; Na(+)/K(+) homeostasis; SOS1 and SKOR genes.