Quantitative Analysis of MOR-1 Internalization in Spinal Cord of Morphine-Tolerant Mice

Vittoria Borgonetti; Nicoletta Galeotti

doi:10.1007/978-1-0716-0884-5_9

Quantitative Analysis of MOR-1 Internalization in Spinal Cord of Morphine-Tolerant Mice

Methods Mol Biol. 2021:2201:99-108. doi: 10.1007/978-1-0716-0884-5_9.

Authors

Vittoria Borgonetti¹, Nicoletta Galeotti²

Affiliations

¹ Department of Neurosciences, Psychology, Drug Research and Child Health (Neurofarba), University of Florence, Florence, Italy.
² Department of Neurosciences, Psychology, Drug Research and Child Health (Neurofarba), University of Florence, Florence, Italy. nicoletta.galeotti@unifi.it.

PMID: 32975792
DOI: 10.1007/978-1-0716-0884-5_9

Abstract

The biological process of opioid analgesic tolerance remains nowadays elusive. In particular the mechanism by which opioid receptor desensitization occurs has not been completely elucidated to date. One possible hypothesis involves the internalization of MOR. Here, we describe a simple in vitro protocol to investigate the localization of MOR-1 after repeated morphine administration in the spinal cord of morphine-tolerant mice, using western blotting and immunofluorescence techniques.

Keywords: Internalization; Morphine; Tolerance; μ-Opioid receptor.

MeSH terms

Analgesics, Opioid / pharmacology
Animals
Blotting, Western / methods*
Drug Tolerance / immunology
Humans
Male
Mice
Mice, Inbred C57BL
Morphine / pharmacology
Receptors, G-Protein-Coupled / genetics
Receptors, G-Protein-Coupled / metabolism
Receptors, Opioid / genetics
Receptors, Opioid / immunology
Receptors, Opioid / metabolism
Receptors, Opioid, mu / genetics
Receptors, Opioid, mu / immunology*
Receptors, Opioid, mu / metabolism
Spinal Cord / immunology*
Spinal Cord / metabolism

Substances

Analgesics, Opioid
Receptors, G-Protein-Coupled
Receptors, Opioid
Receptors, Opioid, mu
Morphine