RP1, a RAGE antagonist peptide, can improve memory impairment and reduce Aβ plaque load in the APP/PS1 mouse model of Alzheimer's disease

Neuropharmacology. 2020 Dec 1:180:108304. doi: 10.1016/j.neuropharm.2020.108304. Epub 2020 Sep 12.

Abstract

Amyloid-β (Aβ) accumulation is a pathological hallmark of Alzheimer's disease (AD). The receptor for advanced glycation end products (RAGE) is involved in the production and accumulation of Aβ. RP1, a peptide antagonist of RAGE, was screened by phage display technology in our previous studies, and its neuroprotective effects on an AD cell model have been confirmed. However, its efficacy in vivo remains unclear. Here, the intranasal delivery of RP1 to APPSwe/PS1dE9 (APP/PS1) mice significantly improved memory impairment and relieved the Aβ burden by decreasing the expression of amyloid precursor protein and β-secretase. RNA-sequencing (RNA-seq) was utilized to identify differentially expressed genes (DEGs) in APP/PS1 mice after RP1 administration. Several DEGs in RAGE downstream signalling pathways were downregulated. Some transcription factors (such as Fos) and the pathways enriched in the remarkable modules may also be related to the efficacy of RP1. In conclusion, RP1 significantly improves the AD symptoms of APP/PS1 mice, and the RNA-seq results provide new ideas for elucidating the possible mechanisms of RP1 treatment.

Keywords: Alzheimer's disease; Amyloid-β; Nasal administration; RNA-sequencing; Receptor for advanced glycation end products (RAGE).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / drug therapy*
  • Alzheimer Disease / genetics
  • Alzheimer Disease / psychology
  • Amyloid beta-Protein Precursor* / genetics
  • Animals
  • Male
  • Maze Learning / drug effects
  • Maze Learning / physiology
  • Memory Disorders / drug therapy*
  • Memory Disorders / genetics
  • Memory Disorders / psychology
  • Mice
  • Mice, Transgenic
  • Microtubule-Associated Proteins / pharmacology
  • Microtubule-Associated Proteins / therapeutic use*
  • Plaque, Amyloid / drug therapy*
  • Plaque, Amyloid / genetics
  • Plaque, Amyloid / psychology
  • Presenilin-1* / genetics
  • Receptor for Advanced Glycation End Products / antagonists & inhibitors*
  • Receptor for Advanced Glycation End Products / genetics

Substances

  • Ager protein, mouse
  • Amyloid beta-Protein Precursor
  • Microtubule-Associated Proteins
  • Presenilin-1
  • RP1 protein, mouse
  • Receptor for Advanced Glycation End Products