Dephosphorylation-directed tricyclic DNA amplification cascades for sensitive detection of protein tyrosine phosphatase

Yueying Li; Shuli Sun; Xiaorui Tian; Jian-Ge Qiu; BingHua Jiang; Li-Juan Wang; Chun-Yang Zhang

doi:10.1039/d0cc04714g

Dephosphorylation-directed tricyclic DNA amplification cascades for sensitive detection of protein tyrosine phosphatase

Chem Commun (Camb). 2020 Oct 1;56(78):11581-11584. doi: 10.1039/d0cc04714g.

Authors

Yueying Li¹, Shuli Sun¹, Xiaorui Tian¹, Jian-Ge Qiu², BingHua Jiang², Li-Juan Wang¹, Chun-Yang Zhang¹

Affiliations

¹ College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China. cyzhang@sdnu.edu.cn wlj-sdnu@foxmail.com.
² Academy of Medical Sciences, Zhengzhou University, Zhengzhou 450000, China.

PMID: 32914789
DOI: 10.1039/d0cc04714g

Abstract

We develop a new fluorescence method for the sensitive detection of protein tyrosine phosphatase 1B (PTP1B) based on dephosphorylation-directed tricyclic DNA amplification cascades. This method exhibits good specificity and high sensitivity with a detection limit of 0.24 pM. Moreover, it can be applied for kinetics analysis, inhibitor screening, and the accurate detection of PTP1B in a variety of cancer cells.

MeSH terms

Cell Line, Tumor
Chymotrypsin / metabolism
DNA / chemistry
DNA / metabolism*
DNA, Catalytic / metabolism
Humans
Limit of Detection
Nucleic Acid Amplification Techniques / methods*
Peptides / chemistry
Peptides / metabolism
Phosphorylation
Protein Tyrosine Phosphatase, Non-Receptor Type 1 / analysis*
Substrate Specificity

Substances

DNA, Catalytic
Peptides
DNA
Protein Tyrosine Phosphatase, Non-Receptor Type 1
Chymotrypsin