Safety of intrathecal delivery of recombinant human arylsulfatase A in children with metachromatic leukodystrophy: Results from a phase 1/2 clinical trial

Mol Genet Metab. 2020 Sep-Oct;131(1-2):235-244. doi: 10.1016/j.ymgme.2020.07.002. Epub 2020 Jul 16.

Abstract

Background: Metachromatic leukodystrophy (MLD) is an autosomal recessive disorder caused by deficient arylsulfatase A (ASA) activity and characterized by neurological involvement that results in severe disability and premature death. We examined the safety and tolerability of intrathecally delivered recombinant human ASA (rhASA; SHP611, now TAK-611) in children with MLD (NCT01510028). Secondary endpoints included change in cerebrospinal fluid (CSF) sulfatide and lysosulfatide levels, and motor function (assessed by Gross Motor Function Measure-88 total score).

Methods: Twenty-four children with MLD who experienced symptom onset aged ≤ 30 months were enrolled. Patients received rhASA every other week (EOW) for 38 weeks at 10, 30, or 100 mg (cohorts 1-3; n = 6 per cohort), or 100 mg manufactured using a revised process (cohort 4; n = 6).

Results: No rhASA-related serious adverse events (SAEs) were observed; 25% of patients experienced an SAE related to the intrathecal device or drug delivery method. Mean CSF sulfatide and lysosulfatide levels fell to within normal ranges in both 100 mg cohorts following treatment. Although there was a general decline in motor function over time, there was a tendency towards a less pronounced decline in patients receiving 100 mg.

Conclusion: Intrathecal rhASA was generally well tolerated at doses up to 100 mg EOW. These preliminary data support further development of rhASA as a therapy for patients with MLD.

Keywords: Arylsulfatase A; Enzyme replacement therapy; Intrathecal; Lysosomal storage disease; Metachromatic leukodystrophy.

Publication types

  • Clinical Trial, Phase I
  • Clinical Trial, Phase II
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Animals
  • Cerebroside-Sulfatase / administration & dosage
  • Cerebroside-Sulfatase / adverse effects
  • Cerebroside-Sulfatase / cerebrospinal fluid
  • Cerebroside-Sulfatase / genetics*
  • Child
  • Child, Preschool
  • Disease Models, Animal
  • Drug-Related Side Effects and Adverse Reactions / genetics
  • Drug-Related Side Effects and Adverse Reactions / pathology
  • Female
  • Genetic Therapy*
  • Humans
  • Infant
  • Injections, Spinal
  • Leukodystrophy, Metachromatic / cerebrospinal fluid
  • Leukodystrophy, Metachromatic / drug therapy*
  • Leukodystrophy, Metachromatic / genetics
  • Leukodystrophy, Metachromatic / pathology
  • Male
  • Recombinant Proteins / administration & dosage
  • Recombinant Proteins / adverse effects
  • Recombinant Proteins / cerebrospinal fluid
  • Recombinant Proteins / genetics*

Substances

  • Recombinant Proteins
  • Cerebroside-Sulfatase

Associated data

  • ClinicalTrials.gov/NCT01510028