Immunohistochemical study of cerebral amyloid angiopathy: use of an antiserum to a synthetic 28-amino-acid peptide fragment of the Alzheimer's disease amyloid precursor

Hum Pathol. 1988 Feb;19(2):214-22. doi: 10.1016/s0046-8177(88)80352-6.

Abstract

A polyclonal antibody to a synthetic peptide representing a 28-amino-acid sequence of the previously isolated and described Alzheimer's disease amyloid precursor was raised in rabbits. The antibody was used in conjunction with an avidin-biotin-peroxidase technique to stain cerebral microvessels involved by amyloid angiopathy and senile "neuritic" plaque amyloid cores. The staining method has significant advantages over standard histologic techniques used to demonstrate brain amyloid and might have important practical applications in the study of microvascular lesions associated with cerebral amyloid angiopathy, as well as in studies on the pathogenesis of Alzheimer's disease or senile dementia of Alzheimer type.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aged
  • Alzheimer Disease / metabolism
  • Amyloid / analysis*
  • Amyloid / immunology*
  • Amyloid beta-Peptides
  • Amyloid beta-Protein Precursor*
  • Amyloidosis / metabolism*
  • Cerebral Cortex / analysis*
  • Cerebrovascular Disorders / metabolism*
  • Humans
  • Immune Sera
  • Immunoenzyme Techniques*
  • Male
  • Peptide Fragments / immunology*
  • Peptides / immunology

Substances

  • Amyloid
  • Amyloid beta-Peptides
  • Amyloid beta-Protein Precursor
  • Immune Sera
  • Peptide Fragments
  • Peptides
  • amyloid beta-protein (1-28)