Boron analysis and imaging of cells with 2-hr BPA exposure by using micro-proton particle-induced gamma-ray emission (PIGE)

Kei Nakai; Keita Endo; Fumiyo Yoshida; Masashi Koka; Naoto Yamada; Takahiro Satoh; Takao Tsurubuchi; Akira Matsumura; Yoshitaka Matsumoto; Hideyuki Sakurai

doi:10.1016/j.apradiso.2020.109334

Boron analysis and imaging of cells with 2-hr BPA exposure by using micro-proton particle-induced gamma-ray emission (PIGE)

Appl Radiat Isot. 2020 Nov:165:109334. doi: 10.1016/j.apradiso.2020.109334. Epub 2020 Jul 23.

Authors

Affiliations

¹ Ibaraki Prefectural University of Health Sciences, 4669-2 Ami, Inashiki, Ibaraki, 300-0331, Japan; Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan; Department of Radiation Oncology, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan. Electronic address: kenakai-nsu@umin.ac.jp.
² Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.
³ Takasaki Advanced Radiation Research Institute, National Institute for Quantum and Radiological Science and Technology, 1233 Watanuki, Takasaki, Gunma, 370-1292, Japan.
⁴ Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan. Electronic address: t-tsurubuchi@md.tsukuba.ac.jp.
⁵ Department of Radiation Oncology, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.

PMID: 32739796
DOI: 10.1016/j.apradiso.2020.109334

Abstract

Particle-induced gamma-ray emission (PIGE), which measures prompt gamma rays at 428 keV from ¹⁰B (p, p'γ) ⁷Be, was used to confirm the boron distribution within 2hr-BPA-exposed cells. Distribution images of potassium, phosphate, and boron and the whole spectrum showed the ratios of boron counts to total (%) as follows: control group: 1.35 ± 0.073%; 2hr boron exposure group: 2.33 ± 0.35%; and boron exposure/wash group: 1.58 ± 0.095%. Micro-beam PIXE/PIGE can be a promising tool for visualization of intracellular Boron.

Keywords: Boron imaging; Boron neutron capture therapy; Boronophenylalanine; Particle induced gamma-ray emission.

MeSH terms

Animals
Boron / analysis*
Boron Neutron Capture Therapy / methods*
Gamma Rays
Spectrum Analysis / methods*

Substances

Boron