Dissemination of Carbapenemases (KPC, NDM, OXA-48, IMP, and VIM) Among Carbapenem-Resistant Enterobacteriaceae Isolated From Adult and Children Patients in China

Renru Han; Qingyu Shi; Shi Wu; Dandan Yin; Mingjia Peng; Dong Dong; Yonggui Zheng; Yan Guo; Rong Zhang; Fupin Hu; China Antimicrobial Surveillance Network (CHINET) Study Group

doi:10.3389/fcimb.2020.00314

Dissemination of Carbapenemases (KPC, NDM, OXA-48, IMP, and VIM) Among Carbapenem-Resistant Enterobacteriaceae Isolated From Adult and Children Patients in China

Front Cell Infect Microbiol. 2020 Jul 3:10:314. doi: 10.3389/fcimb.2020.00314. eCollection 2020.

Authors

Renru Han^{1

2}, Qingyu Shi^{1

2}, Shi Wu^{1

2}, Dandan Yin^{1

2}, Mingjia Peng^{1

2}, Dong Dong^{1

2}, Yonggui Zheng^{1

2}, Yan Guo^{1

2}, Rong Zhang³, Fupin Hu^{1

2}; China Antimicrobial Surveillance Network (CHINET) Study Group

Affiliations

¹ Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China.
² Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai, China.
³ Department of Clinical Laboratory, School of Medicine, Second Affiliated Hospital of Zhejiang University, Hangzhou, China.

Abstract

This study aimed to investigate the dissemination and characteristics of bla_KPC, bla_NDM, bla_OXA-48-like , bla_IMP, and bla_VIM among the carbapenem-resistant Enterobacteriaceae (CRE) strains isolated from adult and children patients. A total of 935 non-duplicate CRE strains were collected from 36 hospitals in 24 provinces or cities across China from 2016 to 2018. Antimicrobial susceptibility testing was performed by broth microdilution method and carbapenemase genes bla_KPC, bla_NDM, bla_OXA-48-like , bla_IMP, and bla_VIM were screened by PCR and confirmed by DNA sequencing. Overall, carbapenemases were produced in 97.4% (911/935) of CRE strains, including KPC-2 (51.6%, 482/935), NDM (35.7%, 334/935), and OXA-48-like carbapenemases (7.3%, 68/935). Overall, the most prevalent carbapenemase gene was bla_KPC-2 among Klebsiella pneumoniae (64.6%, 457/709) and the CRE strains isolated from adult patients (70.3%, 307/437), and bla_NDM among Escherichia coli (96.0%, 143/149) and the CRE strains from children (49.0%, 247/498). The bla_OXA-232-positive carbapenem-resistant K. pneumoniae (9.3%, 66/709) were all isolated from children. Sixteen strains were positive for bla_IMP and 9 strains produced multiple carbapenemases. No strain was positive for bla_VIM. Most of the CRE strains (>90%) were resistant to cephalosporins and carbapenems, more than half (>50%) were resistant to aminoglycosides and fluoroquinolones, but the majority (95.8 and 98.4%) were susceptible to polymyxin B and tigecycline. Ceftazidime-avibactam showed excellent in vitro activity against bla_KPC-2 and bla_OXA-48-like positive strains (100% susceptible). In China, KPC-2, NDM, and OXA-48-like carbapenemases were predominant among CRE clinical isolates. The most prevalent carbapenemase gene was bla_KPC-2 among K. pneumoniae isolates from adult patients, and bla_NDM among E. coli isolates from children.

Keywords: blaIMP; blaKPC-2; blaNDM; blaOXA-48-like; carbapenem-resistant Enterobacteriaceae.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
Carbapenem-Resistant Enterobacteriaceae* / genetics
Child
China / epidemiology
Enterobacteriaceae Infections* / epidemiology
Escherichia coli / genetics
Humans
Microbial Sensitivity Tests
beta-Lactamases / genetics

Substances

Anti-Bacterial Agents
Bacterial Proteins
beta-Lactamases
carbapenemase